Dr Nikki Jordan-Mahy

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Dr Nikki Jordan-Mahy BSc, PhD

BSc/MSci Biochemistry Course Leader and Principal Lecturer


Summary

Dr Nikki Jordan-Mahy is the BSc/MSci Biochemistry Course Leader and Principal Lecturer at Sheffield Hallam University. She is a graduate of Royal Holloway and Bedford New College at the University of London where she received a BSc Hons degree in Physiology and Zoology. On completion of her degree she undertook her PhD at Cambridge University at the MRC Dunn Nutrition Unit. She has taught in many areas including physiology, anatomy, embryology, pathology, forensic pathology and nutrition. She is a member of the Cancer Research Group and the Musculoskeletal and Regenerative Medicine Research Group in the Biomolecular Research Centre (BMRC). Nikki supervises a number of PhD students within these two research areas.

About

Nikki has been nominated for the College and University Inspirational Teaching Award in 2013, 2014, 2015 and 2016 and won the University Award in 2014, 2015 and 2016. She was awarded the VC Inspirational Teaching Award in 2016.

She has worked in the Department of Biosciences and Chemistry for 20 years, and during this time, she has developed modules and course programs of study for undergraduates and postgraduates. She has planned key roles in the seven BSc/MSci course validation processes, in several successful accreditation from the IBMS, and more recently an Advanced Accreditation by the Society of Biology. Nikki has been a Course Leader for five degree programs, and is the current Course Leader for the BSc/MSci in biochemistry degree.

She is the Chair of the College of Health, Wellbeing and Life Sciences Biosciences Research Ethics Committee and has been a member of the University Research Ethics Committee for over 15 years. She works with both University and College members to implement and update ethical procedures and policies in the University.

She also works as an external examiner for Leeds Beckett University.

Nikki is a member of the Biomolecular Sciences Research Centre (BMRC) and part of the Cancer and Musculoskeletal and Regenerative Medicine Research groups.

Cancer Research

Her cancer research focuses on the use of polyphenols in the treatment of cancer, and their impact on standard chemotherapy treatments. This work is in collaboration with Dr. Gordon McDougall from the James Hutton Institute and other members of the BMRC and has led to the production of a number of important publications.

Other cancer research projects involve the study of metastasis formation research with Dr Nick Peak and Dr Neil Cross, and the modulation of the immune system in the treatment of cancer with Dr Rebecca Leyland.

Regenerative Medicine Research

Her regenerative medicine research focuses on the development of Novel 3D Cell culture systems for the study of gastrointestinal disease. This research is in collaboration with Prof Christine Le Maitre from the BMRC and Prof Chris Sammon from MERI. This collaboration has enabled a more materialistic approach to gastrointestinal research.

Further work into epithelial regenerative is also being undertaken in collaboration with Prof Christine Le Maitre from the BMRC and Prof Andrew Alderson from MERI and Prof Prasad Godbole at the Sheffield Children's Hospital.

Specialist areas of interest

Cancer cell biology/biochemistry and regenerative medicine and tissue engineering

Teaching

Biosciences

BSc/MSci Biology
BSc/MSci Biochemistry
BSc Biomedical Sciences
BSc Human Biology

Human Physiology and Anatomy
Human Reproduction and Embryological Development
Professional and Scientific Practice 1 and 2
Control Physiology
BSc Projects
MSci Projects

Research

Research Collaborations

The use of polyphenols in the treatment of leukaemia and their impact on standard chemotherapy treatments. This work is in collaboration with Dr Gordon McDougall from the James Hutton Institute and members of the BMRC.

Other cancer research projects involve the study of metastasis formation research with Dr Nick Peake and Dr Neil Cross, and the modulation of the immune system in the treatment of cancer with Dr Rebecca Leyland.

Investigating the use of Novel 3D Cell culture method for the study and treatments of small intestinal inflammatory disease. This research is in collaboration with Prof Christine Le Maitre from the BMRC and Prof Chris Sammon from MERI. This collaboration has enabled a more materialistic approach to gastrointestinal research.

Epithelial regenerative medicine research. This research is in collaboration with Prof Christine Le Maitre from the BMRC and Prof Andrew Alderson from MERI and Prof Prasad Godbole.

Funding

Epithelial regenerative medicine research. This research is in collaboration with Prof Christine Le Maitre from the BMRC and Prof Andrew Alderson from MERI and Prof Prasad Godbole. Funded by Pioneer, SCH and SHU.

Cancer research collaborators include Dr Gordon McDougall from the James Hutton Institute and Dr Neil Cross, Dr Sarah Haywood-Small and Prof Christine Le Maitre from the BMRC. This work has been funded and sponsored by the Saudi Arabian Government.

Regenerative gastrointestinal medicine research is in collaboration with Prof Christine Le Maitre from the BMRC and Prof Chris Sammon from MERI. This work is funded and sponsored by the Iraqi Government.

Epithelial regenerative medicine research is in collaboration with Prof Christine Le Maitre from the BMRC, Prof Andrew Alderson form MERI and Prof Prasad Godbole from Sheffield Children's Hospital.

Publications

Key Publications

Dosh, R. (2018). Developing models of the small intestine. (Doctoral thesis). Supervised by Le Maitre, C. http://doi.org/10.7190/shu-thesis-00131

Mahbub, A.A.-.H. (2015). The anti-cancer potential of polyphenols in the treatment of leukaemia. (Doctoral thesis). Supervised by Jordan-Mahy, N., Cross, N., Le Maitre, C., & Haywood-Small, S.

Journal articles

Hudson, K., Cross, N.A., Jordan-Mahy, N., & Leyland, R. (2022). Programmed death-ligand 1 expression in human cancer three-dimensional cell culture models. . http://doi.org/10.1101/2022.10.31.514495

Mahbub, A.A., Le Maitre, C., Cross, N., & Jordan-Mahy, N. (2022). The effect of apigenin and chemotherapy combination treatments on apoptosis-related genes and proteins in acute leukaemia cell lines. Scientific Reports, 12 (1). http://doi.org/10.1038/s41598-022-11441-z

Alaswad, H., Mahbub, A., Le Maitre, C., & Jordan-Mahy, N. (2021). Molecular Action of Polyphenols in Leukaemia and Their Therapeutic Potential. International Journal of Molecular Sciences, 22 (6). http://doi.org/10.3390/ijms22063085

Hudson, K., Cross, N., Jordan-Mahy, N., & Leyland, R. (2020). The Extrinsic and Intrinsic Roles of PD-L1 and Its Receptor PD-1: Implications for Immunotherapy Treatment. Frontiers in Immunology, 11, 568931. http://doi.org/10.3389/fimmu.2020.568931

Mardling, P., Alderson, A., Jordan-Mahy, N., & Le Maitre, C. (2020). The use of auxetic materials in tissue engineering. Biomaterials Science. http://doi.org/10.1039/c9bm01928f

Dosh, R., Jordan-Mahy, N., Sammon, C., & Le Maitre, C.L. (2019). Use of l-pNIPAM hydrogel as a 3D-scaffold for intestinal crypts and stem cell tissue engineering. Biomaterials Science, 7 (10), 4310-4324. http://doi.org/10.1039/C9BM00541B

Mahbub, A.A., Le Maitre, C., Haywood-Small, S., Cross, N., & Jordan-Mahy, N. (2019). Polyphenols enhance the activity of alkylating agents in leukaemia cell lines. Oncotarget, 10 (44). http://doi.org/10.18632/oncotarget.27068

Le Maitre, C.L., Dosh, R., Jordan-Mahy, N., & Sammon, C. (2019). Interleukin 1 is a key driver of inflammatory bowel disease-demonstration in a murine IL-1Ra knockout model. Oncotarget. http://doi.org/10.18632/oncotarget.26894

Dosh, R.H., Jordan-Mahy, N., Sammon, C., & Le Maitre, C.L. (2019). Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease. Scientific reports, 9 (1), 1812. http://doi.org/10.1038/s41598-019-38524-8

Dosh, R., Jordan-Mahy, N., Sammon, C., & Le Maitre, C. (2018). Tissue engineering laboratory models of the small intestine. Tissue engineering. Part B, Reviews, 24 (2), 98-111. http://doi.org/10.1089/ten.TEB.2017.0276

Dosh, R., Essa, A., Jordan-Mahy, N., Sammon, C., & Le Maitre, C. (2017). Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium. Acta biomaterialia. http://doi.org/10.1016/j.actbio.2017.08.035

Mahbub, A., Le Maitre, C., Haywood-Small, S., Cross, N., & Jordan-Mahy, N. (2017). Dietary polyphenols influence antimetabolite agents: methotrexate, 6-mercaptopurine and 5-fluorouracil in leukemia cell lines. Oncotarget, 8 (62), 104877-104893. http://doi.org/10.18632/oncotarget.20501

Mahbub, A.A., Le Maitre, C.L., Haywood-Small, S.L., Cross, N.A., & Jordan-Mahy, N. (2015). Glutathione is key to the synergistic enhancement of doxorubicin and etoposide by polyphenols in leukaemia cell lines. Cell Death and Disease, 6, e2028. http://doi.org/10.1038/cddis.2015.379

Mahbub, A.A., Le Maitre, C.L., Haywood-Small, S.L., Cross, N.A., & Jordan-Mahy, N. (2015). Polyphenols act synergistically with doxorubicin and etoposide in leukaemia cell lines. Cell Death Discovery, 1 (15043), 1-12. http://doi.org/10.1038/cddiscovery.2015.43

Dahlawi, H., Jordan-Mahy, N., Clench, M., McDougall, G.J., & Le Maitre, C. (2013). Polyphenols are responsible for the proapoptotic properties of pomegranate juice on leukemia cell lines. Food Science and Nutrition, 1 (2), 196-208. http://doi.org/10.1002/fsn3.26

Mahbub, A.A., Le Maitre, C., Haywood-Small, S., McDougall, G.J., Cross, N., & Jordan-Mahy, N. (2013). Differential effects of polyphenols on proliferation and apoptosis in human myeloid and lymphoid leukemia cell lines. Anti-cancer agents in medicinal chemistry, 13 (10), 1601-1613. http://doi.org/10.2174/18715206113139990303

Phillips, K., Jordan-Mahy, N., Nicklin, M.J.H., & Le Maitre, C. (2013). Interleukin-1 receptor antagonist deficient mice provide insights into pathogenesis of human intervertebral disc degeneration. Annals of the rheumatic diseases, 72 (11), 1860-7. http://doi.org/10.1136/annrheumdis-2012-202266

Dahlawi, H., Jordan-Mahy, N., Clench, M., & Le Maitre, C. (2012). Bioactive actions of pomegranate fruit extracts on leukemia cell lines In vitro hold promise for new therapeutic agents for leukemia. Nutrition and Cancer, 64 (1), 100-110. http://doi.org/10.1080/01635581.2012.630155

McDougall, G.J., Dobson, P., & Jordan-Mahy, N. (2010). Effect of different cooking regimes on rhubarb polyphenols. Food chemistry, 119 (2), 758-764. http://doi.org/10.1016/j.foodchem.2009.07.030

N'Dow, J., Jordan, N., Robson, C.N., Neal, D.E., & Pearson, J.P. (2005). The bladder does not appear to have a dynamic secreted continuous mucous gel layer. The Journal of urology, 173 (6), 2025-2031. http://doi.org/10.1097/01.ju.0000158454.47299.ae

Sherwood, P.V., Wibawa, J.I.D., Atherton, J.C., Jordan, N., Jenkins, D., Barrett, D.A., ... Spiller, R.C. (2002). Impact of acid secretion, gastritis, and mucus thickness on gastric transfer of antibiotics in rats. Gut, 51 (4), 490-495. http://doi.org/10.1136/gut.51.4.490

Newton, J.L., Jordan, N., Pearson, J., Williams, G.V., Allen, A., & James, O.F. (2000). The adherent gastric antral and duodenal mucus gel layer thins with advancing age in subjects infected with Helicobacter pylori. Gerontology, 46 (3), 153-157. http://doi.org/10.1159/000022151

Newton, J.L., Jordan, N., Oliver, L., Strugala, V., Pearson, J., James, O.F., & Allen, A. (1998). Helicobacter pylori in vivo causes structural changes in the adherent gastric mucus layer but barrier thickness is not compromised. Gut, 43 (4), 470-475. http://doi.org/10.1136/gut.43.4.470

Jordan, N., Newton, J., Pearson, J., & Allen, A. (1998). A novel method for the visualization of the in situ mucus layer in rat and man. Clinical science (London, England : 1979), 95 (1), 97-106. http://doi.org/10.1042/cs0950097

JORDAN, N., NEWTON, J., PEARSON, J., & ALLEN, A. (1998). A novel method for the visualization of the in situ mucus layer in rat and man. Clinical Science, 95 (1), 97-106. http://doi.org/10.1042/cs0950097

Strugala, V., Jordan, N., Pearson, J.P., & Allen, A. (1998). The colonic mucus barrier and changes in inflammatory bowel disease. Gastroenterology, 114, A1092. http://doi.org/10.1016/s0016-5085(98)84439-9

Allen, A., Newton, J., Oliver, L., Jordan, N., Strugala, V., Pearson, J.P., & Dettmar, P.W. (1997). Mucus and H. pylori. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society, 48 (3), 297-305.

Oliver, L., Newton, J.L., Jordan, N., Goddard, P., Dettmar, P., Pearson, J.P., & Allen, A. (1997). Effects of Helicobacter pylori colonisation on the adherent gastric mucus barrier. Biochemical Society transactions, 25 (2), 372S. http://doi.org/10.1042/bst025372s

Newton, J., Jordan, N., Williams, G., Allen, A., James, O., & Newton, J. (1997). Thinning of the Gastric Mucus Gel Layer in H Pylori Positive Subjects with Advancing Age. Age and Ageing, 26 (suppl 3), P8. http://doi.org/10.1093/ageing/26.suppl_3.p8-c

Conference papers

Hudson, K., Cross, N., Jordan-Mahy, N., & Leyland, R. (2021). Characterization of 3D models of human breast, prostate and colorectal cancer. CANCER RESEARCH, 81 (13).

Dahlawi, H., Jordan-Mahy, N., & Le Maitre, C. (2015). Antiproliferative and induction of apoptosis by pomegranate's anthocyanidins (delphinidin, cyanidin and pelargonidin) on lymphoid and myelopid leukaemia cell lines. BRITISH JOURNAL OF HAEMATOLOGY, 169, 72-73.

Mahbub, A., Le Maitre, C., Haywood-Small, S.L., McDougall, G., Cross, N.A., & Jordan-Mahy, N. (2012). The Anti-cancerous Potential of Polyphenols in the Treatment of Human Myeloid and Lymphoid Leukaemia. JOURNAL OF PATHOLOGY, 228, S34.

Dahlawi, H., Jordan-Mahy, N., Clench, M.R., McDougall, G.J., & Le Maitre, C. (2012). Impact of Anthocyanin Chemical Structure Found in Pomegranate Juice on Leukaemia Treatment. JOURNAL OF PATHOLOGY, 228, S37.

Wilson, A., Jordan-Mahy, N., Haywood-Small, S., Cross, N.A., & Le Maitre, C. (2012). Extracts From Red and White Cabbage Contains Bio-Active Compounds Which Induce Apoptosis in Myeloid and Lymphoid Leukaemia Cell Lines. JOURNAL OF PATHOLOGY, 228, S37.

Stanley, K.N., Cabry, S.H., Dalton, C.F., & Jordan-Mahy, N. (2007). Use of mucin-overproducing cell line HT29-MTX to study the interaction between Campylobacter jejuni and commensal micro-organisms at the mucin interface of the gastrointestinal tract. ZOONOSES AND PUBLIC HEALTH, 54, 102.

Theses / Dissertations

Alaswad, H. (2024). The Molecular Action of Polyphenols in Leukeamia. (Doctoral thesis). Supervised by Jordan-Mahy, N.

Alzufairi, A.A. (2024). Enhancement of Breast Cancer Chemotherapy and Radiotherapy Responses by Modulation of Ferroptosis. (Doctoral thesis). Supervised by Cross, N., & Jordan-Mahy, N. http://doi.org/10.7190/shu-thesis-00623

Alaswad, H.A. (2024). Molecular Mechanisms of Action of Polyphenols andPolyacetylenes in Leukaemia Cell Lines. (Doctoral thesis). Supervised by Jordan-Mahy, N., Le Maitre, C., & Mahbub, A. http://doi.org/10.7190/shu-thesis-00615

Alzufairi, A. (2024). The Induction of Ferroptosis's in 3D Models of Breast Cancer. (Doctoral thesis). Supervised by Jordan-Mahy, N., & Cross, N.

Hudson, K.V. (2023). Investigating the Intrinsic Role of Programmed Death-Ligand 1 in Human Cancers. (Doctoral thesis). Supervised by Leyland, R., Cross, N., & Jordan-Mahy, N. http://doi.org/10.7190/shu-thesis-00520

Hudson, K.V. (2023). Investigating the Intrinsic Role of Programmed Death-Ligand 1 in Human Cancers. (Doctoral thesis). Supervised by Leyland, R., Cross, N., & Jordan-Mahy, N. http://doi.org/10.7190/shu-thesis-00520

Dahlawi, H. (2013). Effect of pomegranate extracts on apoptosis and cell cycle in haematological malignancies. (Doctoral thesis). Supervised by Le Maitre, C., & Jordan-Mahy, N.

Posters

Hudson, K., Cross, N., Jordan-Mahy, N., & Leyland, R. (2021). Investigating the tumour-intrinsic role of programmed death-ligand 1 in 2D and 3D cell culture models of human breast cancer. Presented at: British Society for Immunology Congress 2021, Edinburgh, UK, 2021

Hudson, K., Cross, N., Jordan-Mahy, N., & Leyland, R. (2020). Blockade of Programmed Death-Ligand 1 with Atezolizumab in Human Breast Cancer 3D Spheroid Colonies Induces Changes in Cell Viability. Presented at: EACR-AACR-ASPIC Conference: Tumour Microenvironment, Lisbon, Portugal, 2020

Hudson, K., Jordan-Mahy, N., Cross, N., & Leyland, R. (2019). Programmed death-ligand 1 expression in human cancer cell lines in two-dimensional and three-dimensional cell culture systems. Presented at: BACR: Tumour Microenvironment meeting, Nottingham, UK, 2019

Hudson, K., Cross, N., Jordan-mahy, N., & Leyland, R. (2018). Flow cytometric phenotyping of diverse human cancer cell lines for immunological biomarkers expression. Presented at: BMRC/MERI Winter Poster Event, Sheffield, 2018

Owens, K., Peake, N., Jordan-Mahy, N., & Leyland, R. (2018). Exploring the interactions of Interferon-gamma and polyphenols in colorectal cancer cells. Presented at: British Society for Immunology: Yorkshire Immunology Group Annual Symposium 2018, University of York, UK, 2018

Other activities

External examiner for Leeds Beckett University

Postgraduate supervision

Rasha Dosh - The development of 3D Models of the intestine

Hamza Alaswad - The use of nutraceuticals in the treatment of leukaemia

Paul Mardling - Tissue engineering

Previous supervision:

Development of tissue engineering models for epithelia tissues

The development of novel 3D cell culture models for the investigation of inflammatory bowel disease

The anti-cancer potential of polyphenols in the treatment of leukaemia

The anti-cancer potential of pomegranate juice extract in the treatment of leukaemia

The adherent colonic mucin layer

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